Abstract Single-molecule localization microscopy requires sparse activation of emitters to circumvent the diffraction limit. In densely labeled or thick samples. overlap of emitter images is inevitable. Single-molecule localization of these samples results in a biased parameter estimate with a wrong model of the number of emitters. https://www.roneverhart.com/100-Percent-Sunglasses-SPEEDCRAFT-LE-Money-Mike-HiPER-Silver-Mirror/