The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products and solutions were digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the https://douglasw100ite2.hamachiwiki.com/user